Application
PKC zeta antibody detects PKC zeta protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKC zeta antibody diluted by 1:1000.Application
Immunoprecipitation of PKC zeta protein from 293T whole cell extracts using 5 μg of PKC zeta antibody . Western blot analysis was performed using PKC zeta antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Product Name | PKC zeta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PKC zeta (protein kinase C, zeta)Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PKC zeta. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Pig, Rabbit, BovineMouse (100%), Rat (100%), Zebrafish (100%), Pig (100%), Rabbit (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | protein kinase C, zeta , PRKCZ , PKC2 , PKC-ZETA |
|---|---|
| Target | PKC zeta |
| Molecular Weight(MW) | 68 kDa (note) (4) |
| Cellular Localization | Cytoplasm , Endosome |
Application
PKC zeta antibody detects PKC zeta protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKC zeta antibody diluted by 1:1000.
Application
Immunoprecipitation of PKC zeta protein from 293T whole cell extracts using 5 μg of PKC zeta antibody . Western blot analysis was performed using PKC zeta antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
