Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKC zeta antibody ().Application
Western blot analysis of extract from 293 cells and Hela using PKC zeta Antibody .| Product Name | PKC zeta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PKC zetaThis is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization. |
| Immunogen | Peptide sequence around aa. 408~412 ( T-S-T-F-C) derived from PKC zeta |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PKC-ZETA, PKC2, PRKCZ, nPKC-zeta, protein kinase C zeta type, protein kinase C, zeta |
|---|---|
| Target | PKC zeta |
| Molecular Weight(MW) | 78 (note) |
Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKC zeta antibody ().
Application
Western blot analysis of extract from 293 cells and Hela using PKC zeta Antibody .| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
