Application
Western blot analysis of PKC-pan phosphorylation expression in NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.| Product Name | PKC-pan (phospho Thr497) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PKC-pan (phospho Thr497)Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthesized peptide derived from human PKC-pan around the phosphorylation site of Thr497 |
| Modification | p-Thr497 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PKC-pan (phospho Thr497) (phospho Thr497), phospho PKC-pan (phospho Thr497) (pT497), PKC-pan (phospho Thr497) Phospho Thr497, PKC-pan (phospho Thr497) T497 Phospho, phospho PKC-pan (phospho Thr497) (Thr497), PK |
|---|---|
| Target | PKC-pan (phospho Thr497) |
| Tissue Specificity | PRKCA |
Application
Western blot analysis of PKC-pan phosphorylation expression in NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.| Application Notes | WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
