Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKM antibody diluted at 1:10000.Application
Immunofluorescence analysis of methanol-fixed A431, using Pyruvate Kinase (muscle) antibody at 1:500 dilution.Application
Pyruvate Kinase (muscle) antibody detects PKM2 protein at cytosol on RT2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded RT2 xenograft. Pyruvate Kinase (muscle) antibody dilution: 1:500.Application
Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE diluted at 1:5000Application
Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKM antibody diluted at 1:5000.| Product Name | PKM antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PKM (muscle) (pyruvate kinase, muscle)The protein encoded by this gene is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Pyruvate Kinase (muscle). The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Rabbit, Chicken, BovineXenopus laevis (86%), Rabbit (97%), Chicken (87%), Bovine (90%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CTHBP antibody, MGC3932 antibody, OIP3 antibody, PK3 antibody, PKM antibody, TCB antibody, THBP1 antibody, PKM2 antibody, PK, muscle type antibody, tumor M2-PK antibody, OIP-3 antibody, pyruvate kinase 2/3 antibody, pyruvate kinase muscle isozyme antibody, |
|---|---|
| Molecular Weight(MW) | 58 kDa (note) (8) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKM antibody diluted at 1:10000.
Application
Immunofluorescence analysis of methanol-fixed A431, using Pyruvate Kinase (muscle) antibody at 1:500 dilution.
Application
Pyruvate Kinase (muscle) antibody detects PKM2 protein at cytosol on RT2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded RT2 xenograft. Pyruvate Kinase (muscle) antibody dilution: 1:500.
Application
Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE diluted at 1:5000
Application
Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKM antibody diluted at 1:5000.| Positive Control | 293T , A431 , mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
