Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PKN beta antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry.Application
Western blot analysis of anti-PKN-beta Pab in HeLa cell lysate. PKNbeta (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.| Product Name | PKN beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PKN betaProtein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol activated/phospholipid dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK). The calcium/calmodulin dependent kinase (CAMK) group consists of 75 kinases regulated by Ca2+/CaM and close relative family (CAMK, CAMKL, DAPK, MAPKAPK). |
| Immunogen | Synthetic peptide conjugated to KLH, corresponding to amino acids 477-492 of Human PKN beta. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| ELISA: Use at a dilution of 1/1000. WB: Use at a dilution of 1/100 - 1/500. Detects a band of approximately 100 kDa (predicted molecular weight: 106 kDa). Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Protein G |
| Gene Synonyms | RP11-545E17.1 |
|---|---|
| Target | PKN beta |
| Cellular Localization | Cytoplasmic |
Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PKN beta antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry.
Application
Western blot analysis of anti-PKN-beta Pab in HeLa cell lysate. PKNbeta (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.| Positive Control | HeLa cell lysate |
|---|---|
| Application Notes | ELISA: Use at a dilution of 1/1000. WB: Use at a dilution of 1/100 - 1/500. Detects a band of approximately 100 kDa (predicted molecular weight: 106 kDa). Optimal dilutions/concentrations should be determined by the end user. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.09% Sodium Azide; Constituents: PBS |
