Application
PKNOX1 antibody immunoprecipitates PKNOX1 ?protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of PKNOX1 ?protein by 2 μg of PKNOX1 ?antibody 10% SDS-PAGE The immunoprecipitated PKNOX1 ?protein was detected by PKNOX1 ?antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.Application
Sample (30 ug of whole cell lysate) A: HepG2 B: HepG2 nucleus 10% SDS PAGE diluted at 1:2000Application
PKNOX1 antibody detects PKNOX1 protein at nucleus by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PKNOX1 protein stained by PKNOX1 antibody diluted at 1:1000. Blue: Hoechst 33342 staining.| Product Name | PKNOX1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PKNOX1 (PBX/knotted 1 homeobox 1) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PKNOX1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWBChIP |
| Not tested in other applications. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rhesus Monkey, BovineMouse (94%), Rat (94%), Rhesus Monkey (99%), Bovine (91%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PREP1 antibody, pkonx1c antibody, PKNOX1 antibody, human homeobox-containing protein antibody, homeobox protein PREP-1 antibody, Pbx regulating protein-1 antibody, PBX/knotted homeobox 1 antibody, homeobox protein PKNOX1 antibody, PBX/knotted 1 homeobox 1 |
|---|---|
| Molecular Weight(MW) | 48 kDa (note) (4) |
| Cellular Localization | Nucleus |
Application
PKNOX1 antibody immunoprecipitates PKNOX1 ?protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of PKNOX1 ?protein by 2 μg of PKNOX1 ?antibody 10% SDS-PAGE The immunoprecipitated PKNOX1 ?protein was detected by PKNOX1 ?antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Application
Sample (30 ug of whole cell lysate) A: HepG2 B: HepG2 nucleus 10% SDS PAGE diluted at 1:2000
Application
PKNOX1 antibody detects PKNOX1 protein at nucleus by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PKNOX1 protein stained by PKNOX1 antibody diluted at 1:1000. Blue: Hoechst 33342 staining.| Positive Control | HepG2 nucleus |
|---|---|
| Application Notes | Not tested in other applications. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
