Application
Western blot analysis of PKNOX2 expression in HeLa (A); mouse kidney (B); rat kidney (C) whole cell lysates.Application
Immunohistochemical analysis of PKNOX2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Product Name | PKNOX2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PKNOX2Homeodomain proteins are sequence-specific transcription factors that share a highly conserved DNA-binding domain and play fundamental roles in cell proliferation, differentiation, and death. PKNOX2 belongs to the TALE (3-amino acid loop extension) class of homeodomain proteins characterized by a 3-amino acid extension between alpha helices 1 and 2 within the homeodomain (Imoto et al., 2001 [PubMed 11549286]).[supplied by OMIM, Oct 2009] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PKNOX2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIHC-PWB |
| Species Reactivity | HumanMouseChickenRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PREP2 , PBX/knotted 1 homeobox 2 |
|---|---|
| Target | PKNOX2 |
| Tissue Specificity | Recognizes endogenous levels of PKNOX2 protein. |
Application
Western blot analysis of PKNOX2 expression in HeLa (A); mouse kidney (B); rat kidney (C) whole cell lysates.
Application
Immunohistochemical analysis of PKNOX2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 C. Upon delivery aliquot and store at -20 C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide. |
