Application
Western blot analysis of PKR (pT451) expression in HeLa PMA-treated (A); Raw264.7 H2O2-treated (B); NIH3T3 LPS-treated (C) whole cell lysates.Application
Immunohistochemical analysis of PKR (pT451) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Product Name | PKR (phospho Thr451) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PKR (phospho Thr451)The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PKR (pT451). The exact sequence is proprietary. |
| Modification | p-Thr451 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PRKR , eukaryotic translation initiation factor 2-alpha kinase 2 , PKR , EIF2AK1 , EIF2AK2 |
|---|---|
| Target | PKR (phospho Thr451) |
| Tissue Specificity | Recognizes endogenous levels of PKR (pT451) protein. |
Application
Western blot analysis of PKR (pT451) expression in HeLa PMA-treated (A); Raw264.7 H2O2-treated (B); NIH3T3 LPS-treated (C) whole cell lysates.
Application
Immunohistochemical analysis of PKR (pT451) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Application Notes | WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide. |
