Application
Immunofluorescence staining of methanol-fixed Hela cells using PKR Antibody .Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PKR Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from 293, Hela and C6 cells using PKR(Ab-446) Antibody| Product Name | PKR antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PKRFollowing activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection. |
| Immunogen | Peptide sequence around aa.444~448 (K-R-T-R-S) derived from Human PKR. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | EIF2AK1, MGC126524, PKR, PRKR, EIF2AK2, tyrosine-protein kinase EIF2AK2, eIF-2A protein kinase 2, protein kinase, interferon-inducible double stranded RNA dependent, interferon-induced, double-stranded RNA-activated protein kinase, double stranded RNA acti |
|---|---|
| Target | PKR |
| Molecular Weight(MW) | 68 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using PKR Antibody .
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PKR Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from 293, Hela and C6 cells using PKR(Ab-446) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
