Application
PKR antibody detects PKR protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKR antibody diluted at a dilution of 1:1000.Application
Immunoprecipitation of PKR protein from MCF-7 whole cell extracts using 5 μg of PKR antibody . Western blot analysis was performed using PKR antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
PKR antibody detects PKR protein at cytoplasm in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. PKR antibody diluted at 1:500.| Product Name | PKR antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PKREIF2AK2 is a protein serine/threonine kinase that acquires enzymatic activity following autophosphorylation, a process mediated by double-stranded RNA (dsRNA). Activation of EIF2AK2 allows the kinase to phosphorylate its natural substrate, the alpha subunit of eukaryotic protein synthesis initiation factor-2 (EIF2-alpha; MIM 603907), leading to the inhibition of protein synthesis (summary by Kuhen et al., 1996 [PubMed 8812437]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PKR. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Rhesus Monkey, ChimpanzeeRhesus Monkey (82%), Chimpanzee (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PRKR , eukaryotic translation initiation factor 2-alpha kinase 2 , EIF2AK2 , EIF2AK1 |
|---|---|
| Target | PKR |
| Molecular Weight(MW) | 62 kDa (note) (3) |
Application
PKR antibody detects PKR protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKR antibody diluted at a dilution of 1:1000.
Application
Immunoprecipitation of PKR protein from MCF-7 whole cell extracts using 5 μg of PKR antibody . Western blot analysis was performed using PKR antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
PKR antibody detects PKR protein at cytoplasm in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. PKR antibody diluted at 1:500.| Positive Control | MCF-7 , MDA-MB-231 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
