Rabbit Polyclonal antibody to PKRThe protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Immunogen
PKR antibody was raised against a peptide corresponding to 14 amino acids near the amino terminus of human PKR.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
ELISAIHC-PWB
WB: 1 - 2 ug/ml. IHC-P: 5 ug/ml
Species Reactivity
HumanRat
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Western blot analysis of PKR in MCF7 cell lysate with PKR antibody at (A) 1 and (B) 2 ug/mL.
Application
Immunohistochemistry of PKR in rat lung tissue with PKR antibody at 5 ug/mL.
Application Notes
WB: 1 - 2 ug/ml. IHC-P: 5 ug/ml
Additional Information
Form
Liquid
Storage Instructions
Be stored at 4oC for three months and -20oC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.