Application
Immunohistochemical analysis of paraffin-embedded human lung cancer, using PLA2G4A antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE diluted at 1:3000Application
PLA2G4A antibody detects PLA2G4A protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: PLA2G4A protein stained by PLA2G4A antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | PLA2G4A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PLA2G4A (phospholipase A2, group IVA (cytosolic, calcium-dependent))This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PLA2G4A. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Rabbit, Chicken, BovineMouse (92%), Rat (93%), Xenopus laevis (81%), Rabbit (92%), Chicken (84%), Bovine (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC126350 antibody, PLA2G4 antibody, cPLA2-alpha antibody, PLA2G4A antibody, phospholipase A2 group IVA antibody, calcium-dependent phospholipid-binding protein antibody, cytosolic phospholipase A2 antibody, phosphatidylcholine 2-acylhydrolase antibody, ly |
|---|---|
| Molecular Weight(MW) | 85 kDa (note) (3) |
| Cellular Localization | Cytoplasm , Cytoplasmic vesicle |
Application
Immunohistochemical analysis of paraffin-embedded human lung cancer, using PLA2G4A antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE diluted at 1:3000
Application
PLA2G4A antibody detects PLA2G4A protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: PLA2G4A protein stained by PLA2G4A antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | A549 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
