| Product Name | Placental Alkaline Phosphatase antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Placental Alkaline PhosphataseThere are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized. [provided by RefSeq] |
| Immunogen | Purified PLAP from human placenta |
| Clonality | Polyclonal |
|---|---|
| Isotype | Cocktail |
| Host Species | Rabbit |
| Tested Applications | IHC-FIHC-PIPWB |
| Immunoblot 1:100-1:500. Immunoprecipitation, Immunohistochemistry 1:50. The optimal conditions should be determined by the individual laboratory. This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 with AutoProbe III (GTX28620). It is recommended that this product be used on frozen tissue sections or specimens. The optimal conditions should be determined by the individual laboratory. | |
| Species Reactivity | HumanMonkeyMouseRat |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ALP, FLJ61142, PALP, PLAP |
|---|---|
| Target | Placental alkaline phosphatase |
| Tissue Specificity | Reacts with human PLAP specifically as determined by immunodiffusion in an agarose plate. |
| Positive Control | Third Trimester Placenta |
|---|---|
| Application Notes | Immunoblot 1:100-1:500. Immunoprecipitation, Immunohistochemistry 1:50. The optimal conditions should be determined by the individual laboratory. This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 with AutoProbe III (GTX28620). It is recommended that this product be used on frozen tissue sections or specimens. The optimal conditions should be determined by the individual laboratory. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing 0.2% BSA and 0.09% sodium azide |
