Application
Human Seminoma stained with anti-PLAP antibodyApplication
Human Placenta stained with anti-PLAP antibody| Product Name | Placental Alkaline Phosphatase antibody [SP15] (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Monoclonal Rabbit Antibody to Human PLAP |
| Immunogen | Recombinant protein encoding human Placental Alkaline Phosphatase (PLAP). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | SP15 |
| Host Species | Rabbit |
| Tested Applications | IHCIHC-P |
| We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Target | PLAP |
|---|---|
| Tissue Specificity | This antibody reacts with a membrane-bound isoenzyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. This antibody is highly specific to PLAP and shows no cross-reaction with other isoenzymes of alkaline phosphatases. It is useful in the identification of testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA).This antibody crossreacts with Human, Mouse and Rat. |
| Cellular Localization | Cytoplasmic |
Application
Human Seminoma stained with anti-PLAP antibody
Application
Human Placenta stained with anti-PLAP antibody| Positive Control | Placenta |
|---|---|
| Application Notes | We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
