Application
Sample (30 µg of whole cell lysate) A: Jurkat B: Raji C: K562 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Plasminogen antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Plasminogen antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of methanol-fixed HeLa, using Plasminogen antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using Plasminogen antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using Plasminogen antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: BCL-1 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Plasminogen antibody detects PLG protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 7.5% SDS-PAGE Plasminogen antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Plasminogen antibody detects Plasminogen protein at cytoplasm in rat prostate by immunohistochemical analysis. Sample: Paraffin-embedded rat prostate. Plasminogen antibody diluted at 1:500.Application
Human tissue extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Plasminogen antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Plasminogen antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Plasminogen (plasminogen)Plasminogen (PLG) is a circulating zymogen that is converted to the active enzyme plasmin by cleavage of the peptide bond between arg560 and val561, which is mediated by urokinase (PLAU; MIM 191840) and tissue plasminogen activator (PLAT; MIM 173370). The main function of plasmin is to dissolve fibrin (see, e.g., FGA, MIM 134820) clots. Plasmin, like trypsin, belongs to the family of serine proteinases (Miyata et al., 1982 [PubMed 6216475]; Forsgren et al., 1987 [PubMed 3030813]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Plasminogen. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Dog, Rhesus Monkey, BovineDog (81%), Rhesus Monkey (93%), Bovine (81%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp779M0222 antibody, PLG antibody, plasminogen antibody, plasmin antibody |
|---|---|
| Molecular Weight(MW) | 91 kDa (note) |
| Cellular Localization | Secreted |
Application
Sample (30 µg of whole cell lysate) A: Jurkat B: Raji C: K562 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Plasminogen antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Plasminogen antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using Plasminogen antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using Plasminogen antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using Plasminogen antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: BCL-1 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Plasminogen antibody detects PLG protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 7.5% SDS-PAGE Plasminogen antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Plasminogen antibody detects Plasminogen protein at cytoplasm in rat prostate by immunohistochemical analysis. Sample: Paraffin-embedded rat prostate. Plasminogen antibody diluted at 1:500.
Application
Human tissue extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Plasminogen antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | Jurkat , Raji , K562 , BCL-1 , PC-12 , Rat2 , human plasma , mouse plasma , rat plasma |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
