Application
Plastin L antibody detects Plastin L protein at cell membrane and cytoplasm in mouse thymus gland by immunohistochemical analysis. Sample: Paraffin-embedded mouse thymus gland. Plastin L antibody diluted at 1:500.Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with L-Plastin antibody diluted at 1:10000.Application
L-Plastin antibody detects L-Plastin protein at cytoplasm and focal adhesion site by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: L-Plastin protein stained by L-Plastin antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | Plastin L antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Plastin L (lymphocyte cytosolic protein 1 (L-plastin))Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). However, L-plastin has been found in many types of malignant human cells of non-hemopoietic origin suggesting that its expression is induced accompanying tumorigenesis in solid tissues. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Plastin L. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Chicken, Rhesus Monkey, BovineRat (97%), Zebrafish (86%), Xenopus laevis (88%), Chicken (92%), Rhesus Monkey (99%), Bovine (97%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CP64 antibody, DKFZp781A23186 antibody, FLJ25423 antibody, FLJ26114 antibody, FLJ39956 antibody, L-PLASTIN antibody, LC64P antibody, LPL antibody, PLS2 antibody, LCP1 antibody, L-plastin (Lymphocyte cytosolic protein 1) (LCP-1) (LC64P) antibody, bA139H14.1 |
|---|---|
| Molecular Weight(MW) | 70 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
Plastin L antibody detects Plastin L protein at cell membrane and cytoplasm in mouse thymus gland by immunohistochemical analysis. Sample: Paraffin-embedded mouse thymus gland. Plastin L antibody diluted at 1:500.
Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with L-Plastin antibody diluted at 1:10000.
Application
L-Plastin antibody detects L-Plastin protein at cytoplasm and focal adhesion site by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: L-Plastin protein stained by L-Plastin antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | A549 , H1299 , HCT116 , Raji , K562 , THP-1 , HL-60 , NIH-3T3 , JC , BCL-1 |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
