Application
Immunofluorescence staining of methanol-fixed Hela cells using PLC-g1(phospho-Tyr771) AntibodyApplication
Western blot analysis of extracts from 3T3 cells untreated or treated with serum using PLC-g1(phospho-Tyr771) Antibody| Product Name | PLCG1 (phospho Tyr771) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PLCG1 (phospho Tyr771)PLC-gamma is a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 771 (P-D-Y(p)-G-A) derived from Human PLC- gamma1. |
| Modification | p-Tyr771 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | NCKAP3, PLC-II, PLC1, PLC148, PLCgamma1, PLCG1, 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1, phosphatidylinositol phospholipase C, phosphoinositide phospholipase C, phosphoinositidase C, phospholipase C-II, phospholipase C, gamma 1 (f |
|---|---|
| Target | PLC gamma1 (Phospho Tyr771) |
| Molecular Weight(MW) | 155 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using PLC-g1(phospho-Tyr771) Antibody
Application
Western blot analysis of extracts from 3T3 cells untreated or treated with serum using PLC-g1(phospho-Tyr771) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
