Application
Immunofluorescent staining using Phospholipase C γ-1 [pY783] Polyclonal Antibody, Rabbit: PLC-1 gamma [pY783] phosphospecific antibody. Colocalized detection of PLC-1 gamma [pY783] (green) and actin stress fibers (red) at the sites of membrane ruffles and focal adhesions in fibroblasts.| Product Name | PLCG1 (phospho Tyr783) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to PLCG1 (phospho Tyr783)The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | Synthetic peptide (Human) derived from the region of Phospholipase C gamma that contains tyrosine 783, based on the human sequence. This region is conserved among many species including human, rat, bovine and frog (Xenopus laevis). |
| Modification | p-Tyr783 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB: Use at a dilution of 1/1000. Predicted molecular weight: 135 kDa. Can be blocked with GTX25249 Phospholipase C gamma 1 phospho Y783 and non-phospho peptides (pair) or GTX25354 NIH3T3 whole cell lysate, + / - PDGF stimulation (pair) Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanRatXenopusBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Phospholipase C1 gamma (Phospho Y783) , Phospholipase C1 gamma Tyr783 phospho , Phospholipase C1 gamma phospho Tyr783 , PLCGAMMA1 , phospho Tyr783 Phospholipase C1 gamma , phospho Phospholipase C1 gamma Tyr783 , PLCG1 , Phospholipase C1 gamma (Phosp |
|---|---|
| Target | Phospholipase C1 gamma |
| Tissue Specificity | Phosphorylation site-specific antibody selective for the phosphorylated form of the Phospholipase C gamma 1 containing a phosphate on tyrosine 783. The antibody has been shown to recognize the endogenous form of Phospholipase C gamma when phosphorylated on tyrosine 783 in a variety of cell types following treatment by a broad range of extracellular stimuli. Applications include NIH 3T3 cells +/- PDGF. The antibody does not react with a site directed mutant (Y783F). |
Application
Immunofluorescent staining using Phospholipase C γ-1 [pY783] Polyclonal Antibody, Rabbit: PLC-1 gamma [pY783] phosphospecific antibody. Colocalized detection of PLC-1 gamma [pY783] (green) and actin stress fibers (red) at the sites of membrane ruffles and focal adhesions in fibroblasts.| Application Notes | WB: Use at a dilution of 1/1000. Predicted molecular weight: 135 kDa. Can be blocked with GTX25249 Phospholipase C gamma 1 phospho Y783 and non-phospho peptides (pair) or GTX25354 NIH3T3 whole cell lysate, + / - PDGF stimulation (pair) Optimal dilutions/concentrations should be determined by the end user. |
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: 50% Glycerol, PBS, 1mg/ml BSA (IgG, protease free). pH 7.3 |
