Application
Western blot analysis of PLC gamma 2 (pY1217) expression in HEK293T EGF-treated (A); A431 (B); NIH3T3 (C); SP2/0 EGF-treated (D); H9C2 EGF-treated (E) whole cell lysates.| Product Name | PLCG2 (phospho Tyr1217) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PLCG2 (phospho Tyr1217)The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3. [provided by RefSeq, Mar 2014] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PLC gamma 2. The exact sequence is proprietary. |
| Modification | p-Tyr1217 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FCAS3 , PLCG2 , APLAID , phospholipase C, gamma 2 (phosphatidylinositol-specific) , PLC-IV , PLC-gamma-2 |
|---|---|
| Target | PLC gamma 2 (phospho Tyr1217) |
| Tissue Specificity | Recognizes endogenous levels of PLC gamma 2 (pY1217) protein. |
Application
Western blot analysis of PLC gamma 2 (pY1217) expression in HEK293T EGF-treated (A); A431 (B); NIH3T3 (C); SP2/0 EGF-treated (D); H9C2 EGF-treated (E) whole cell lysates.| Application Notes | WB: 1:500 - 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide. |
