Application
WB analysis of extracts from untreated and EGF-treated HepG2 cells using PLC gamma 2 (phospho Tyr753) antibody.| Product Name | PLCG2 (phospho Tyr753) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to PLCG2 (phospho Tyr753)The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG), using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. [provided by RefSeq, Sep 2011] |
| Immunogen | The antiserum was produced against synthesized phosphopeptide derived from human PLC gamma 2 around the phosphorylation site of tyrosine 753. |
| Modification | p-Tyr753 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB: 1:500-1:1000 IF:1:100-1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PLCG2 , APLAID , FCAS3 , phospholipase C, gamma 2 (phosphatidylinositol-specific) |
|---|---|
| Target | PLC gamma 2 (phospho Tyr753) |
| Tissue Specificity | This antibody detects endogenous levels of PLC gamma 2 only when phosphorylated at tyrosine 753. |
Application
WB analysis of extracts from untreated and EGF-treated HepG2 cells using PLC gamma 2 (phospho Tyr753) antibody.| Application Notes | WB: 1:500-1:1000 IF:1:100-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
