Application
Western blot analysis of PLC gamma 2 (pY753) expression in Hela TNFa-treated (A); MCF7 (B); Raw264.7 TNFa-treated (C); mouse kidney (D); rat kidney (E) whole cell lysates.Application
Immunohistochemical analysis of PLC gamma 2 (pY753) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Product Name | PLCG2 (phospho Tyr753) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PLCG2 (phospho Tyr753)The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3. [provided by RefSeq, Mar 2014] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PLC gamma 2. The exact sequence is proprietary. |
| Modification | p-Tyr753 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | APLAID , phospholipase C, gamma 2 (phosphatidylinositol-specific) , PLC-IV , PLC-gamma-2 , FCAS3 , PLCG2 |
|---|---|
| Target | PLC gamma 2 (phospho Tyr753) |
| Tissue Specificity | Recognizes endogenous levels of PLC gamma 2 (pY753) protein. |
Application
Western blot analysis of PLC gamma 2 (pY753) expression in Hela TNFa-treated (A); MCF7 (B); Raw264.7 TNFa-treated (C); mouse kidney (D); rat kidney (E) whole cell lysates.
Application
Immunohistochemical analysis of PLC gamma 2 (pY753) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Application Notes | WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide. |
