Application
Immunofluorescence analysis of methanol-fixed HeLa, using PLC gamma 2 antibody at 1:200 dilution.Application
Sample (30 µg of whole cell lysate) A: Raji 5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using PLCG2 antibody at 1:100 dilution.Application
PLCG2 antibody [N1N2], N-term detects PLCG2 protein by western blot analysis. A. 30 µg BCL-1 whole cell lysate/extract 5% SDS-PAGE PLCG2 antibody [N1N2], N-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | PLCG2 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PLCG2 (phospholipase C, gamma 2 (phosphatidylinositol-specific))Enzymes of the phospholipase C family catalyze the hydrolysis of phospholipids to yield diacylglycerols and water-soluble phosphorylated derivatives of the lipid head groups. A number of these enzymes have specificity for phosphoinositides. Of the phosphoinositide-specific phospholipase C enzymes, C-beta is regulated by heterotrimeric G protein-coupled receptors, while the closely related C-gamma-1 (PLCG1; MIM 172420) and C-gamma-2 enzymes are controlled by receptor tyrosine kinases. The C-gamma-1 and C-gamma-2 enzymes are composed of phospholipase domains that flank regions of homology to noncatalytic domains of the SRC oncogene product, SH2 and SH3.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human PLCG2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | RatRat (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PLCG2 antibody, phospholipase C-IV antibody, phospholipase C gamma 2 antibody, phospholipase C, gamma 2 (phosphatidylyinositol-specific) antibody, phospholipase C-gamma-2 antibody, phosphoinositide phospholipase C-gamma-2 antibody, PLC-IV antibody, PLC-gam |
|---|---|
| Molecular Weight(MW) | 148 kDa (note) |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using PLC gamma 2 antibody at 1:200 dilution.
Application
Sample (30 µg of whole cell lysate) A: Raji 5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using PLCG2 antibody at 1:100 dilution.
Application
PLCG2 antibody [N1N2], N-term detects PLCG2 protein by western blot analysis. A. 30 µg BCL-1 whole cell lysate/extract 5% SDS-PAGE PLCG2 antibody [N1N2], N-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | Raji , BCL-1 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
