Western blot analysis of extracts from HepG2 cells using PLD1 (Phospho-Thr147) Antibody. The lane on the right is treated with the antigen-specific peptide.
Rabbit Polyclonal antibody to PLD1 (phospho Thr147)This gene encodes a phosphatidylcholine-specific phospholipase which catalyzes the hydrolysis of phosphatidylcholine in order to yield phosphatidic acid and choline. The enzyme may play a role in signal transduction and subcellular trafficking. Alternative splicing results in multiple transcript variants with both catalytic and regulatory properties. [provided by RefSeq, Sep 2011]
Immunogen
Peptide sequence around phosphorylation site of threonine 147(R-H-T(p)-F-R) derived from Human PLD1.
Modification
p-Thr147
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
WB: 1:500 - 1:1000
Species Reactivity
Human
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
The antibody detects endogenous levels of PLD1 only when phosphorylated at threonine 147.
Application
Application
Western blot analysis of extracts from HepG2 cells using PLD1 (Phospho-Thr147) Antibody. The lane on the right is treated with the antigen-specific peptide.
Application Notes
WB: 1:500 - 1:1000
Additional Information
Form
Liquid
Storage Instructions
Store at -20˚C/1 year
Storage Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.