Application
Pleckstrin antibody [C2C3], C-term detects PLEK protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in -20℃ 100% MeOH for 5 min. Green: PLEK protein stained by Pleckstrin antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33343 staining.Application
Sample(30 μg of whole cell lysate) A:Raji 12% SDS PAGE diluted at 1:500| Product Name | Pleckstrin antibody [C2C3], C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Pleckstrin (pleckstrin)Major protein kinase C substrate of platelets, its exact function is not known. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Pleckstrin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IF,WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, BovineMouse (93%), Rat (93%), Dog (93%), Bovine (93%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ27168 antibody, P47 antibody, PLEK antibody, platelet 47 kDa protein antibody, pleckstrin antibody |
|---|---|
| Molecular Weight(MW) | 40 kDa (note) |
Application
Pleckstrin antibody [C2C3], C-term detects PLEK protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in -20℃ 100% MeOH for 5 min. Green: PLEK protein stained by Pleckstrin antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33343 staining.
Application
Sample(30 μg of whole cell lysate) A:Raji 12% SDS PAGE diluted at 1:500| Positive Control | Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
