Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLEK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLEK.Application
HEK293T cells transfected with either RC203780 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PLEK antibody, and then analyzed by flow cytometry.| Product Name | PLEK antibody [4G6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [4G6] to PLEKMajor protein kinase C substrate of platelets, its exact function is not known. |
| Immunogen | Full length human recombinant protein of human PLEK (NP_002665) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 4G6 |
| Host Species | Mouse |
| Tested Applications | FACSWB |
| Flow Cytometry 1:100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | P47 |
|---|---|
| Target | PLEK |
| Tissue Specificity | is specific for human PLEK (NP_002665). |
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLEK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLEK.
Application
HEK293T cells transfected with either RC203780 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PLEK antibody, and then analyzed by flow cytometry.| Application Notes | Flow Cytometry 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
