Application
Western blot analysis of HCT-116 lysate using PLK1 antibody at 1:2000.Application
IHC analysis of human placenta using PLK1 antibody at 1:100.Application
Western Blot: STPK14 Antibody [ - Western blot analysis of PLK1 in HCT-116 using NB100-56651 at 1:2000.| Product Name | PLK1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PLK1Polo related kinase (PLK), a serine/threonine kinase is strongly correlated with the mitotic activity of cells. PLK is present in all tissues during mouse embryonic development, where as in adults expresion is limited to thymus and ovaries. It has been observed that tumors of various origin (lung, colon, stomach, smooth muscle, and esophagus as well as non-Hodgkin lymphomas) express high levels of PLK transcripts with absent of transcripts in normal surrounding tissues. Only normal colon and placenta, which are known to be mitotically active express PLK. PLK expression is restricted to proliferating cells. In mammalian cells, three PLKs (PLK1, PLK2/Snk, PLK3/Fnk) have been identified. PLK1 is the most studied isoform. The known substrates of PLK1 include several proteins involved in mitosis such as Cdc25C, APC, and cyclin B. PLK1 promotes mitosis through the phosphorylation and activation of Cdc25C, which regulates the tyrosine dephosphorylation of CDKs. |
| Immunogen | A portion of amino acids 100-200 of human PLK1 was used as the immunogen. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIHC-PIPWBChIP |
| Western Blot 1:1000-1:2000 | |
| Species Reactivity | HumanMouseDogPigRatZebra FishC.elegansPrimateXenopusBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | plk1, Polo-Like Kinase 1 antibody, PoloLike Kinase 1 antibody, PoloLike Kinase1 antibody, PLK, STPK13, Polo Like Kinase 1 antibody |
|---|---|
| Target | PLK1 |
| Tissue Specificity | The amino acid sequence used as immunogen is 100% homologous in human, mouse, rat, pig, monkey, and dog, and 92% homologous in C. elegans, zebrafish and Xenopus. |
Application
Western blot analysis of HCT-116 lysate using PLK1 antibody at 1:2000.
Application
IHC analysis of human placenta using PLK1 antibody at 1:100.
Application
Western Blot: STPK14 Antibody [ - Western blot analysis of PLK1 in HCT-116 using NB100-56651 at 1:2000.| Application Notes | Western Blot 1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.05% BSA |
