Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PLK4 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
PLK4 antibody detects PLK4 protein at cytoplasm and nucleus in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. PLK4 antibody diluted at 1:500.Application
PLK4 antibody detects PLK4 protein at cytoplasm and nucleus in human endometrial cancer by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial cancer. PLK4 antibody diluted at 1:500.Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using PLK4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Product Name | PLK4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PLK4 (polo-like kinase 4)This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human PLK4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | BovineBovine (87%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | SAK antibody, STK18 antibody, PLK4 antibody, serine/threonine kinase 18 antibody, serine/threonine-protein kinase 18 antibody, serine/threonine protein kinase SAK antibody, Snk akin kinase antibody, serine/threonine-protein kinase PLK4 antibody, serine/thr |
|---|---|
| Molecular Weight(MW) | 109 kDa (note) (3) |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PLK4 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
PLK4 antibody detects PLK4 protein at cytoplasm and nucleus in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. PLK4 antibody diluted at 1:500.
Application
PLK4 antibody detects PLK4 protein at cytoplasm and nucleus in human endometrial cancer by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial cancer. PLK4 antibody diluted at 1:500.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using PLK4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
