Application
Immunoprecipitation of PMS2 protein from HeLa whole cell extracts using 5 μg of PMS2 antibody [N1N3] . Western blot analysis was performed using PMS2 antibody [N1N3] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
PMS2 antibody [N1N3] detects PMS2 protein by western blot analysis. A. 30 μg 293T whole cell extract B. 30 μg whole cell extract of human PMS2-transfected 293T cells 7.5 % SDS-PAGE PMS2 antibody [N1N3] dilution: 1:5000Application
PMS2 antibody [N1N3] detects PMS2 protein by western blot analysis. A. 30 μg A431 whole cell extract B. 30 μg HeLa whole cell extract C. 30 μg HepG2 whole cell extract 7.5 % SDS-PAGE PMS2 antibody [N1N3] dilution: 1:1000| Product Name | PMS2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to PMS2 (PMS2 postmeiotic segregation increased 2 (S. cerevisiae))This gene is one of the PMS2 gene family members found in clusters on chromosome 7. The product of this gene is involved in DNA mismatch repair. It forms a heterodimer with MLH1 and this complex interacts with other complexes bound to mismatched bases. Mutations in this gene are associated with hereditary nonpolyposis colorectal cancer, Turcot syndrome, and are a cause of supratentorial primitive neuroectodermal tumors. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human PMS2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, BovineMouse (88%), Bovine (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HNPCC4 antibody PMS2CL antibody PMSL2 antibody PMS2 antibody H_DJ0042M02.9 antibody mismatch repair endonuclease PMS2 antibody DNA mismatch repair protein PMS2 antibody PMS1 protein homolog 2 antibody PMS2 postmeiotic segregation increased 2 (S. ce |
|---|---|
| Target | PMS2 |
| Molecular Weight(MW) | 96 kDa (note) (4) |
| Cellular Localization | Nucleus |
Application
Immunoprecipitation of PMS2 protein from HeLa whole cell extracts using 5 μg of PMS2 antibody [N1N3] . Western blot analysis was performed using PMS2 antibody [N1N3] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
PMS2 antibody [N1N3] detects PMS2 protein by western blot analysis. A. 30 μg 293T whole cell extract B. 30 μg whole cell extract of human PMS2-transfected 293T cells 7.5 % SDS-PAGE PMS2 antibody [N1N3] dilution: 1:5000
Application
PMS2 antibody [N1N3] detects PMS2 protein by western blot analysis. A. 30 μg A431 whole cell extract B. 30 μg HeLa whole cell extract C. 30 μg HepG2 whole cell extract 7.5 % SDS-PAGE PMS2 antibody [N1N3] dilution: 1:1000| Positive Control | A431 , HeLa , HepG2 , human PMS2-transfected 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
