Application
PNK antibody detects PNK protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg whole cell lysate/extract of human PNK-transfected 293T cells 7.5 % SDS-PAGE PNK antibody dilution: 1:20000| Product Name | PNK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PNK (polynucleotide kinase 3'-phosphatase)This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PNK. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, RatMouse (83%), Rat (82%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | polynucleotide kinase 3'-phosphatase, MCSZ, PNKP, EIEE10 |
|---|---|
| Target | PNK |
| Molecular Weight(MW) | 57 kDa (note) |
| Cellular Localization | Nucleus |
Application
PNK antibody detects PNK protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg whole cell lysate/extract of human PNK-transfected 293T cells 7.5 % SDS-PAGE PNK antibody dilution: 1:20000| Positive Control | human PNK-transfected 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
