Application
Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE diluted at 1:2000Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft , using PNK antibody at 1:500 dilution.| Product Name | PNK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PNK (polynucleotide kinase 3'-phosphatase)This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PNK. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, RatMouse (82%), Rat (81%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | EIEE10 antibody, MCSZ antibody, PNK antibody, PNKP antibody, DNA 5'-kinase/3'-phosphatase antibody, Homo sapiens polynucleotide kinase 3'-phosphatase (PNKP) antibody, bifunctional polynucleotide phosphatase/kinase antibody, polynucleotide kinase 3'-phospha |
|---|---|
| Molecular Weight(MW) | 57 kDa (note) (2) |
Application
Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE diluted at 1:2000
Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft , using PNK antibody at 1:500 dilution.| Positive Control | A549 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
