Application
Sample (15 ug of whole cell lysate) A: HUVEC 12% SDS PAGE diluted at 1:5000Application
Immunofluorescence analysis of methanol-fixed HCT116, using POFUT1 antibody at 1:500 dilution.Application
Sample (50 ug of whole cell lysate) A: mouse liver 10% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE diluted at 1:1000| Product Name | POFUT1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to POFUT1 (protein O-fucosyltransferase 1)This gene encodes a member of the glycosyltransferase O-Fuc family. This enzyme adds O-fucose through an O-glycosidic linkage to conserved serine or threonine residues in the epidermal growth factor-like repeats of a number of cell surface and secreted proteins. O-fucose glycans are involved in ligand-induced receptor signaling. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human POFUT1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Dog, Pig, Chimpanzee, BovineRat (97%), Dog (93%), Pig (95%), Chimpanzee (100%), Bovine (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FUT12 antibody KIAA0180 antibody MGC2482 antibody O-FUT antibody O-Fuc-T antibody O-FucT-1 antibody POFUT1 antibody peptide-O-fucosyltransferase 1 antibody GDP-fucose protein O-fucosyltransferase 1 antibody o-fucosyltransferase protein antibody p |
|---|---|
| Molecular Weight(MW) | 44 kDa (note) (3) |
| Cellular Localization | Endoplasmic reticulum |
Application
Sample (15 ug of whole cell lysate) A: HUVEC 12% SDS PAGE diluted at 1:5000
Application
Immunofluorescence analysis of methanol-fixed HCT116, using POFUT1 antibody at 1:500 dilution.
Application
Sample (50 ug of whole cell lysate) A: mouse liver 10% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE diluted at 1:1000| Positive Control | HCT116 , mouse liver , HUVEC , HUVEC treat with Sphingosine 1-phosphate |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
