Application
Western blot analysis of DNA Polymerase delta 3 expression in HeLa (A); MCF7 (B); Jurkat (C); mouse brain (D) whole cell lysates.| Product Name | POLD3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to POLD3This gene encodes the 66-kDa subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein plays a role in regulating the activity of DNA polymerase delta through interactions with other subunits and the processivity cofactor proliferating cell nuclear antigen (PCNA). Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Mar 2012] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human DNA Polymerase delta 3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | P66 P68 polymerase (DNA-directed) delta 3 accessory subunit |
|---|---|
| Target | POLD3 |
| Tissue Specificity | Recognizes endogenous levels of DNA Polymerase delta 3 protein. |
Application
Western blot analysis of DNA Polymerase delta 3 expression in HeLa (A); MCF7 (B); Jurkat (C); mouse brain (D) whole cell lysates.| Application Notes | IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 C. Upon delivery aliquot and store at -20 C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide. |
