Rabbit polyclonal antibody to POLDIP3Is involved in regulation of translation. Is preferentially associated with CBC-bound spliced mRNA-protein complexes during the pioneer round of mRNA translation. Contributes to enhanced translational efficiency of spliced over nonspliced mRNAs. Recruits activated ribosomal protein S6 kinase beta-1 I/RPS6KB1 to newly synthesized mRNA.
Immunogen
The antiserum was produced against synthesized peptide derived from human POLDIP3.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
ELISA: 1:40000
Optimal dilutions should be determined experimentally by the end user.
Species Reactivity
HumanMouse
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
Polymerase delta interacting protein 3, Polymerase deltainteracting protein3, POLDIP3, Polymerase deltainteracting protein 3, Polymerase delta-interacting protein 3, Polymerase delta interacting protein3, PDP3, p46, PDIP46, 46 kDa DNA polymerase delta inte
Target
POLDIP3
Molecular Weight(MW)
46 kDa (note)
Tissue Specificity
POLDIP3 Antibody detects endogenous levels of total POLDIP3 protein.
Cellular Localization
Nucleocytoplasmic shuttling protein.
Application
Application
Western blot analysis of extracts from RAW264.7 cells, using POLDIP3 Antibody. The lane on the right is treated with the synthesized peptide.
Application Notes
ELISA: 1:40000
Optimal dilutions should be determined experimentally by the end user.
Additional Information
Form
Liquid
Storage Instructions
Store at -20 degrees Celcius. Product is stable for at least 1 year.
Storage Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.