Application
POLE antibody detects POLE protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: POLE protein stained by POLE antibody diluted at 1:2500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Application
POLE antibody detects POLE protein at nucleus in human ovary by immunohistochemical analysis. Sample: Paraffin-embedded human ovary. POLE antibody diluted at 1:500.Application
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with POLE antibody diluted at 1:1000.| Product Name | POLE antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to POLE (polymerase (DNA directed), epsilon, catalytic subunit)Participates in DNA repair and in chromosomal DNA replication. |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human POLE. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (90%), Rat (90%), Bovine (94%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | POLE1 FILS CRCS12 polymerase (DNA directed) epsilon catalytic subunit |
|---|---|
| Target | POLE |
| Molecular Weight(MW) | 262 kDa (note) |
| Cellular Localization | Nucleus |
Application
POLE antibody detects POLE protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: POLE protein stained by POLE antibody diluted at 1:2500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Application
POLE antibody detects POLE protein at nucleus in human ovary by immunohistochemical analysis. Sample: Paraffin-embedded human ovary. POLE antibody diluted at 1:500.
Application
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with POLE antibody diluted at 1:1000.| Positive Control | K562 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
