Application
WB analysis of extracts from various cells using POLH antibody.Application
IHC-P analysis of human kidney tissue using POLH antibody at a dilution of 1:200.Application
IHC-P analysis of rat brain tissue using POLH antibody at a dilution of 1:200.Application
ICC/IF analysis of U2OS cells using POLH antibody (red) and DAPI (blue).| Product Name | POLH antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to POLHThis gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA; when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant protein of human POLH |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | RAD30 polymerase (DNA directed) eta XP-V RAD30A XPV |
|---|---|
| Target | POLH |
| Molecular Weight(MW) | 78 kDa (note) |
Application
WB analysis of extracts from various cells using POLH antibody.
Application
IHC-P analysis of human kidney tissue using POLH antibody at a dilution of 1:200.
Application
IHC-P analysis of rat brain tissue using POLH antibody at a dilution of 1:200.
Application
ICC/IF analysis of U2OS cells using POLH antibody (red) and DAPI (blue).| Application Notes | WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
