Application
POLR2C antibody [N1C3-2] detects POLR2C protein at nucleus on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. POLR2C antibody [N1C3-2] dilution: 1:500.Application
POLR2C antibody [N1C3-2] detects POLR2C protein at nucleus on mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. POLR2C antibody [N1C3-2] dilution: 1:500.Application
POLR2C antibody [N1C3-2] detects POLR2C protein at nucleus on rat middle brain by immunohistochemical analysis. Sample: Paraffin-embedded rat middle brain. POLR2C antibody [N1C3-2] dilution: 1:500.Application
POLR2C antibody [N1C3-2] detects POLR2C protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. POLR2C antibody [N1C3-2] dilution: 1:500.Application
POLR2C antibody [N1C3-2] detects POLR2C protein at nucleus on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. POLR2C antibody [N1C3-2] dilution: 1:500.Application
Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE diluted at 1:1000Application
POLR2C antibody [N1C3-2] detects POLR2C protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: POLR2C protein stained by POLR2C antibody [N1C3-2] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | POLR2C antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to POLR2C (polymerase (RNA) II (DNA directed) polypeptide C, 33kDa)This gene encodes the third largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a cysteine rich region and exists as a heterodimer with another polymerase subunit, POLR2J. These two subunits form a core subassembly unit of the polymerase. A pseudogene has been identified on chromosome 21. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human POLR2C. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Chicken, Rhesus Monkey, BovineZebrafish (92%), Xenopus laevis (94%), Chicken (94%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | RPB3 antibody RPB31 antibody hRPB33 antibody hsRPB3 antibody POLR2C antibody DNA-directed RNA polymerase II 33 kDa polypeptide antibody DNA-directed RNA polymerase II subunit C antibody DNA-directed RNA polymerase II subunit RPB3 antibody RNA polym |
|---|---|
| Molecular Weight(MW) | 31 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
POLR2C antibody [N1C3-2] detects POLR2C protein at nucleus on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. POLR2C antibody [N1C3-2] dilution: 1:500.
Application
POLR2C antibody [N1C3-2] detects POLR2C protein at nucleus on mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. POLR2C antibody [N1C3-2] dilution: 1:500.
Application
POLR2C antibody [N1C3-2] detects POLR2C protein at nucleus on rat middle brain by immunohistochemical analysis. Sample: Paraffin-embedded rat middle brain. POLR2C antibody [N1C3-2] dilution: 1:500.
Application
POLR2C antibody [N1C3-2] detects POLR2C protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. POLR2C antibody [N1C3-2] dilution: 1:500.
Application
POLR2C antibody [N1C3-2] detects POLR2C protein at nucleus on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. POLR2C antibody [N1C3-2] dilution: 1:500.
Application
Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE diluted at 1:1000
Application
POLR2C antibody [N1C3-2] detects POLR2C protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: POLR2C protein stained by POLR2C antibody [N1C3-2] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | 293T , A431 , Jurkat |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
