Application
Western blot analysis of POLR2I expression in A549 (A); NIH3T3 (B); PC12 (C) whole cell lysates.| Product Name | POLR2I antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to POLR2IThis gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. The product of this gene has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human POLR2I. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseChickenDogMonkeyPigRatRabbitBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | hRPB14.5 polymerase (RNA) II (DNA directed) polypeptide I 14.5kDa RPB9 |
|---|---|
| Target | POLR2I |
| Tissue Specificity | Recognizes endogenous levels of POLR2I protein. |
Application
Western blot analysis of POLR2I expression in A549 (A); NIH3T3 (B); PC12 (C) whole cell lysates.| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 C. Upon delivery aliquot and store at -20 C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide. |
