Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using POLR3E antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: Jurakt 7.5% SDS PAGE diluted at 1:1000| Product Name | POLR3E antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to POLR3E (DNA-directed RNA polymerase III subunit RPC5)DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Essential for efficient transcription from both the type 2 VAI and type 3 U6 RNA polymerase III promoters. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human POLR3E. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevisMouse (96%), Rat (96%), Zebrafish (82%), Xenopus laevis (80%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | RPC5 antibody SIN antibody POLR3E antibody RNA polymerase III subunit C5 antibody DNA-directed RNA polymerase III subunit RPC5 antibody DNA-directed RNA polymerase III 80 kDa polypeptide antibody RNA polymerase III 80 kDa subunit RPC5 antibody polym |
|---|---|
| Molecular Weight(MW) | 80 kDa (note) (6) |
| Cellular Localization | Nucleus |
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using POLR3E antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: Jurakt 7.5% SDS PAGE diluted at 1:1000| Positive Control | Jurakt |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
