Rabbit Polyclonal antibody to POLR3F (polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa)The protein encoded by this gene is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase. [provided by RefSeq]
Immunogen
Recombinant protein encompassing a sequence within the center region of human POLR3F. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
*Optimal dilutions/concentrations should be determined by the researcher.
:
Species Reactivity
Human
Predicted Reactivity
Mouse, Rat, Zebrafish, Chicken, Rhesus Monkey, BovineMouse (100%), Rat (100%), Zebrafish (91%), Chicken (96%), Rhesus Monkey (100%), Bovine (100%)
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
MGC13517 antibody RPC39 antibody RPC6 antibody POLR3F antibody DNA-directed RNA polymerase III subunit F antibody DNA-directed RNA polymerase III subunit RPC6 antibody DNA-directed RNA polymerases III 39 kDa polypeptide antibody RNA polymerase III C
Molecular Weight(MW)
36 kDa (note)
Cellular Localization
Nucleus
Application
Application
Sample (30 ug of whole cell lysate) A: HeLa nucleus 10% SDS PAGE diluted at 1:1000
Positive Control
HeLa nucleus
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher.
:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.