Application
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using POM121 antibody at 1:500 dilution.Application
Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE diluted at 1:2000Application
Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE diluted at 1:2000Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using POM121 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:500.Application
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using POM121 antibody at 1:500 dilution.| Product Name | POM121 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to POM121 (POM121 membrane glycoprotein (rat))The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. This gene encodes a member of the FG-repeat-containing nucleoporins. The protein encoded by this gene is an integral membrane protein that localizes to the central spoke ring complex and participates in anchoring the nuclear pore complex to the nuclear envelope. Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only one has been determined. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human POM121. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | RatRat (81%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp586G1822 antibody DKFZp586P2220 antibody FLJ41820 antibody KIAA0618 antibody MGC3792 antibody POM121A antibody POM121 antibody P145 antibody pore membrane protein of 121 kDa antibody nuclear envelope pore membrane protein POM 121 antibody nu |
|---|---|
| Molecular Weight(MW) | 128 kDa (note) (2) |
| Cellular Localization | Nucleus , nuclear pore complex , Nucleus membrane; Single-pass membrane protein , Endoplasmic reticulum membrane; Single-pass membrane protein (By similarity) |
Application
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using POM121 antibody at 1:500 dilution.
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE diluted at 1:2000
Application
Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE diluted at 1:2000
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using POM121 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:500.
Application
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using POM121 antibody at 1:500 dilution.| Positive Control | A549 , HeLa , HepG2 , HCT116 , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
