Application
Immunohistochemical analysis of paraffin-embedded N87 xenograft, using PON2 antibody at 1:100 dilution.Application
Sample (30 µg of whole cell lysate) A: A431 10% SDS PAGE PON2 antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (50 µg of whole cell lysate) A: mouse liver 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
PON2 antibody [N1C2] detects PON2 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded A549 xenograft. PON2 antibody [N1C2] diluted at 1:500.| Product Name | PON2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PON2 (paraoxonase 2)This gene encodes a member of the paraoxonase gene family, which includes three known members located adjacent to each other on the long arm of chromosome 7. The encoded protein is ubiquitously expressed in human tissues, membrane-bound, and may act as a cellular antioxidant, protecting cells from oxidative stress. Hydrolytic activity against acylhomoserine lactones, important bacterial quorum-sensing mediators, suggests the encoded protein may also play a role in defense responses to pathogenic bacteria. Mutations in this gene may be associated with vascular disease and a number of quantitative phenotypes related to diabetes. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PON2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Dog, Pig, Sheep, BovineRat (90%), Dog (91%), Pig (93%), Sheep (91%), Bovine (92%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PON2 antibody paraoxonase nirs antibody PON 2 antibody aromatic esterase 2 antibody A-esterase 2 antibody serum aryldialkylphosphatase 2 antibody serum paraoxonase/arylesterase 2 antibody paraoxonase 2 antibody |
|---|---|
| Molecular Weight(MW) | 39 kDa (note) (2) |
| Cellular Localization | Membrane; Peripheral membrane protein |
Application
Immunohistochemical analysis of paraffin-embedded N87 xenograft, using PON2 antibody at 1:100 dilution.
Application
Sample (30 µg of whole cell lysate) A: A431 10% SDS PAGE PON2 antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (50 µg of whole cell lysate) A: mouse liver 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
PON2 antibody [N1C2] detects PON2 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded A549 xenograft. PON2 antibody [N1C2] diluted at 1:500.| Positive Control | A431 , H1299 , HeLa , HepG2 , mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
