Application
Wild-type (WT) and POR knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with POR antibody [N2C2], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:1000Application
POR antibody [N2C2], Internal detects Cytochrome P450 Reductase protein at endoplasmic reticulum on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. POR antibody [N2C2], Internal dilution: 1:5| Product Name | POR antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to POR (P450 (cytochrome) oxidoreductase)This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human POR. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Dog, Pig, Rabbit, Chicken, Rhesus Monkey, Bovine, Guinea pigMouse (95%), Rat (95%), Xenopus laevis (84%), Dog (97%), Pig (96%), Rabbit (96%), Chicken (90%), Rhesus Monkey (100%), Bovine (94%), Guinea pig (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CPR antibody CYPOR antibody DKFZp686G04235 antibody FLJ26468 antibody P450R antibody POR antibody NADPH--cytochrome P450 reductase antibody NADPH-dependent cytochrome P450 reductase antibody P450 (cytochrome) oxidoreductase antibody |
|---|---|
| Target | Cytochrome P450 Reductase |
| Molecular Weight(MW) | 77 kDa (note) |
| Cellular Localization | Endoplasmic reticulum membrane; Peripheral membrane protein |
Application
Wild-type (WT) and POR knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with POR antibody [N2C2], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:1000
Application
POR antibody [N2C2], Internal detects Cytochrome P450 Reductase protein at endoplasmic reticulum on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. POR antibody [N2C2], Internal dilution: 1:5| Positive Control | HeLaS3 , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
