Application
Zebrafish embryos extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Pou5f3 antibody diluted at 1:1000.| Product Name | Pou5f3 (Oct4) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Pou5f3 (Oct4) (POU domain, class 5, transcription factor 3)Involved in early development of embryos, especially in the process of gastrulation. May play an important role in establishing and specifying rhombomeric segments. Seems to be required to maintain the cells in a highly undifferentiated state. In contrast to POU2, T-POU2 lacks DNA-binding activity because of its incomplete pou domain structure. Overexpression of POU2 does not have any effect on development, whereas overexpression of t-POU2 causes developmental retardation or arrest before gastrulation. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of zebrafish Pou5f3 (Oct4). The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Zebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | pou2 gp9 pou5f1 oct4 POU-2 wu:fd18d06 POU domain class 5 transcription factor 1 chunp6868 cb197 spg/pou2 gp-9 |
|---|---|
| Target | Pou5f3 (Oct4) |
| Molecular Weight(MW) | 52 kDa (note) |
| Cellular Localization | Nucleus |
Application
Zebrafish embryos extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Pou5f3 antibody diluted at 1:1000.| Positive Control | 2dpf zebrafish embryos |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
