Mouse monoclonal antibody [k1B1] to PP2C betaThe protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase has been shown to dephosphorylate cyclin-dependent kinases (CDKs), and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to cause cell-growth arrest or cell death. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional transcript variants have been described, but currently do not represent full-length sequences. [provided by RefSeq, Jul 2008]
Immunogen
Recombinant human PP2C beta (1-192aa) purified from E. coli
Key Feature
Clonality
Monoclonal
Isotype
IgG2
Clone Number
k1B1
Host Species
Mouse
Tested Applications
ELISAWB
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 3,000. Recommended starting dilution is 1:2,000.:
Species Reactivity
Human
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
protein phosphatase Mg2+/Mn2+ dependent 1B PP2CB PP2C-beta-X PPC2BETAX PP2CBETA
Target
PPM1B
Light Chain Type
kappa
Application
Application
WB analysis of MCF7 lysate (30ug) using PP2C beta antibody at a dilution of 1:2,000.
Application Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 3,000. Recommended starting dilution is 1:2,000.:
Additional Information
Form
Liquid
Storage Instructions
Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
Storage Buffer
Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.