Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PPA1 antibody at 1:200 dilution.Application
Sample (30 µg of whole cell lysate) A: H1299 B: HeLa C: HepG2 D: Molt-4 12% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
PPA1 antibody detects PPA1 protein at cytoplasm in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. PPA1 antibody diluted at 1:500.Application
PPA1 antibody detects PPA1 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract D. 30 µg NIH-3T3 whole cell lysate/extract E. 30 µg BCL-1 whole cell lysate/extract F. 30 µg Raw264.7 whole cell lysate/extract G. 30 µg C2C12 whole cell lysate/extract 12% SDS-PAGE PPA1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
PPA1 antibody detects PPA1 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 12% SDS-PAGE PPA1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | PPA1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PPA1 (pyrophosphatase (inorganic) 1)The protein encoded by this gene is a member of the inorganic pyrophosphatase (PPase) family. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Studies of a similar protein in bovine suggested a cytoplasmic localization of this enzyme. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PPA1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | BovineBovine (95%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | IOPPP antibody MGC111556 antibody PP antibody PP1 antibody SID6-8061 antibody PPA1 antibody pyrophosphate phospho-hydrolase antibody cytosolic inorganic pyrophosphatase antibody inorganic pyrophosphatase 1 antibody inorganic diphosphatase antibody |
|---|---|
| Target | PPA1 |
| Molecular Weight(MW) | 33 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PPA1 antibody at 1:200 dilution.
Application
Sample (30 µg of whole cell lysate) A: H1299 B: HeLa C: HepG2 D: Molt-4 12% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
PPA1 antibody detects PPA1 protein at cytoplasm in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. PPA1 antibody diluted at 1:500.
Application
PPA1 antibody detects PPA1 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract D. 30 µg NIH-3T3 whole cell lysate/extract E. 30 µg BCL-1 whole cell lysate/extract F. 30 µg Raw264.7 whole cell lysate/extract G. 30 µg C2C12 whole cell lysate/extract 12% SDS-PAGE PPA1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
PPA1 antibody detects PPA1 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 12% SDS-PAGE PPA1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | H1299 , HeLa , HepG2 , Molt-4 , Neuro 2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
