Application
Western blot analysis of PPAPDC1B in EL4 cell lysate with PPAPDC1B antibody at (A) 1 and (B) 2 ug/ml.| Product Name | PPAPDC1B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to PPAPDC1BPhosphatidate phosphatase (PAP) plays important role in lipid-signaling metabolism in eukaryotic cells. Two distinct types of PAP (PAP1 and PAP2) activity have been distinguished by their subcellular localization and differential sensitivity to N-ethylmaleimide (NEM) and Mg2+. A yeast diacylglycerol pyrophosphate (DGPP) phosphatase (DPP1) and mammalian DGPP phosphatase (PAP2) have been identified as Mg2+-independent and NEM-insensitive membrane-associated. PPAPDC1A (also known as DPPL2) and PPAPDC1B (DPPL1) form a novel type of Mg2+-independent and NEM-sensitive mammalian phosphatidate phosphatase showing broad substrate specificity. Knockdown experiments indicated that this protein is involved with multiple cell signaling pathways, including the JAK-Stat3, MAP kinase, and PKC pathways. PPAPDC1B may also potentiate the estrogen receptor pathway by down-regulating DUSP22. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DPPL1 phosphatidic acid phosphatase type2 domain containing1B PPAPDC1B PPAPDC1 phosphatidic acid phosphatase type 2 domain containing 1B |
|---|---|
| Target | PPAPDC1B |
| Tissue Specificity | This antibody is specific for PPAPDC1B |
Application
Western blot analysis of PPAPDC1B in EL4 cell lysate with PPAPDC1B antibody at (A) 1 and (B) 2 ug/ml.| Positive Control | Blocking Peptide: Cat.No. GTX85223-PEP - PPAPDC1B Peptide Lysates: Cat. No. GTX27183 - EL4 Cell Lysate |
|---|---|
| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.02% sodium azide |
