Application
Immunofluorescence Microscopy of Rabbit anti-PPAR alpha antibody . Tissue: HepG2 cells. Fixation: 4% formaldehyde fixed (10 min). Antigen retrieval: not required. Primary antibody: PPAR alpha antibody at 1 µg/mL overnight at 4°C. Secondary antibody: Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (green) used at a 1:1000, Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1:200 dilution for 1h for 45 min at RT. Localization: PPAR alpha is nuclear and occasionally cytoplasmic. Staining: PPAR alpha as green fluorescent signal with DAPI (blue) nuclear counterstain.Application
Immunohistochemistry showing GeneTex's PPAR alpha antibody staining of PPAR alpha protein in mouse liver tissue section (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before enzymatic antigen retrieval with 0.05% protease in PBS for 5 minutes. Sample was then blocked with 5% serum for 20 minutes at 20°C. The primary antibody was diluted 1:50 and incubated with sample in Tris plus 5% normal goat serum for 1 hour at 20°C. A biotinylated goat polyclonal to rabbit IgG was used at dilution at 1:500 as secondary antibody. Images show nuclear staining in hepatocytes (perfusion-fixed mouse, 10 and 40x microscope magnification).Application
Affinity Purified anti-PPAR alpha (N -terminal specific) (Rabbit) is shown to detect a 52 kDa band corresponding to PPAR alpha present in a 3T3 whole cell lysate. Approximately 20 µg of lysate was loaded per lane for SDS-PAGE. Detection occurred after using a 1:500 (lane 1) or 1:1000 (lane 2) dilution of antibody followed by 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.Application
Immunohistochemistry using GeneTex's anti-PPAR antibody, showing staining of PPAR alpha in rat brain sections, highlighting cytoplasmic staining in ependymal cells and neurons in frontal cortex. Bottom image shows subventricular zone (svz) of lateral ventrical (exit point of progenitor olfactory neurones); top image shows frontal cortex in the same section. Cytoplasmic staining is also observed in the corpus callosum (bottom image) and in dendritic fields of the cortex. Formalin/PFA-fixed paraffin-embedded sections of rat brain tissue were incubated with the primary antibody at 1:200 for 1 hour. Antigen retrieval was performed by heat induction in citrate buffer pH 6.0.WB
(A) SIRT2 knockdown blocked PPARγ and LXRα, promoted PPARα. The GCs were transfected with SIRT2-siRNA 4 plasmids for 48 h and treated with the SIRT2 inhibitors Thiomyristoyl (2 μM) and SirReal2 (5 μM) for 24 h. Western blotting for PPARα, PPARγ, and LXRα are shown. According to the western blot results, the protein ratios were analyzed in treatment groups relative to the GAPDH protein abundance.(B) SIRT2 knockdown increased Ac-PPARα. Coimmunoprecipitation (coIP) for Ac-PPARα and Ac-PPARγ are shown by using Ac-Lys antibodies. The immunoprecipitates were analyzed by western blotting with the indicated antibodies. IP, immunoprecipitation.| Product Name | PPAR alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PPAR alphaThe Peroxisome Proliferator-Activated Receptors (PPARs) are members of the nuclear hormone receptor subfamily of transcription factors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. |
| Immunogen | Synthetic peptide: MVDTESPICPLSPLEADD, corresponding to N terminal amino acids 1-18 of Mouse PPAR alpha. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAFACSICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | BovineDogHamsterMousePigRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PPAR PPARA NR1C1 4933429D07RIK PPARALPHA AW742785 |
|---|---|
| Target | PPAR Alpha |
| Cellular Localization | Nuclear |
Application
Immunofluorescence Microscopy of Rabbit anti-PPAR alpha antibody . Tissue: HepG2 cells. Fixation: 4% formaldehyde fixed (10 min). Antigen retrieval: not required. Primary antibody: PPAR alpha antibody at 1 µg/mL overnight at 4°C. Secondary antibody: Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (green) used at a 1:1000, Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1:200 dilution for 1h for 45 min at RT. Localization: PPAR alpha is nuclear and occasionally cytoplasmic. Staining: PPAR alpha as green fluorescent signal with DAPI (blue) nuclear counterstain.
Application
Immunohistochemistry showing GeneTex's PPAR alpha antibody staining of PPAR alpha protein in mouse liver tissue section (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before enzymatic antigen retrieval with 0.05% protease in PBS for 5 minutes. Sample was then blocked with 5% serum for 20 minutes at 20°C. The primary antibody was diluted 1:50 and incubated with sample in Tris plus 5% normal goat serum for 1 hour at 20°C. A biotinylated goat polyclonal to rabbit IgG was used at dilution at 1:500 as secondary antibody. Images show nuclear staining in hepatocytes (perfusion-fixed mouse, 10 and 40x microscope magnification).
Application
Affinity Purified anti-PPAR alpha (N -terminal specific) (Rabbit) is shown to detect a 52 kDa band corresponding to PPAR alpha present in a 3T3 whole cell lysate. Approximately 20 µg of lysate was loaded per lane for SDS-PAGE. Detection occurred after using a 1:500 (lane 1) or 1:1000 (lane 2) dilution of antibody followed by 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.
Application
Immunohistochemistry using GeneTex's anti-PPAR antibody, showing staining of PPAR alpha in rat brain sections, highlighting cytoplasmic staining in ependymal cells and neurons in frontal cortex. Bottom image shows subventricular zone (svz) of lateral ventrical (exit point of progenitor olfactory neurones); top image shows frontal cortex in the same section. Cytoplasmic staining is also observed in the corpus callosum (bottom image) and in dendritic fields of the cortex. Formalin/PFA-fixed paraffin-embedded sections of rat brain tissue were incubated with the primary antibody at 1:200 for 1 hour. Antigen retrieval was performed by heat induction in citrate buffer pH 6.0.
WB
(A) SIRT2 knockdown blocked PPARγ and LXRα, promoted PPARα. The GCs were transfected with SIRT2-siRNA 4 plasmids for 48 h and treated with the SIRT2 inhibitors Thiomyristoyl (2 μM) and SirReal2 (5 μM) for 24 h. Western blotting for PPARα, PPARγ, and LXRα are shown. According to the western blot results, the protein ratios were analyzed in treatment groups relative to the GAPDH protein abundance.(B) SIRT2 knockdown increased Ac-PPARα. Coimmunoprecipitation (coIP) for Ac-PPARα and Ac-PPARγ are shown by using Ac-Lys antibodies. The immunoprecipitates were analyzed by western blotting with the indicated antibodies. IP, immunoprecipitation.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. |
| Storage Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide |
