Application
Cross-linked ChIP was performed with HepG2 chromatin extract and 5 μg of either control rabbit IgG or anti-PPAR alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to C3 promotor or NOD1 gene.Application
PPAR alpha antibody immunoprecipitates PPAR alpha protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : Control with 2.5 μg of pre-immune rabbit IgG B : Immunoprecipitation of PPAR alpha by 2.5 μg of PPAR alpha antibody 10% SDS-PAGE The immunoprecipitated PPAR alpha protein was detected by PPAR alpha antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.Application
Sample (30 µg of whole cell lysate) A: Rat primary hepatocyte 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | PPAR alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PPAR alpha (peroxisome proliferator-activated receptor alpha)Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human PPAR alpha. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWBChIP |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRatQueen BeeHoneybee |
| Predicted Reactivity | Dog, Pig, Rhesus MonkeyDog (91%), Pig (92%), Rhesus Monkey (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC2237 antibody MGC2452 antibody NR1C1 antibody PPAR antibody PPARalpha antibody hPPAR antibody PPARA antibody peroxisome proliferator-activated receptor alpha antibody PPAR-alpha antibody peroxisome proliferator-activated nuclear receptor alpha |
|---|---|
| Molecular Weight(MW) | 52 kDa (note) (2) |
Application
Cross-linked ChIP was performed with HepG2 chromatin extract and 5 μg of either control rabbit IgG or anti-PPAR alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to C3 promotor or NOD1 gene.
Application
PPAR alpha antibody immunoprecipitates PPAR alpha protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : Control with 2.5 μg of pre-immune rabbit IgG B : Immunoprecipitation of PPAR alpha by 2.5 μg of PPAR alpha antibody 10% SDS-PAGE The immunoprecipitated PPAR alpha protein was detected by PPAR alpha antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
Application
Sample (30 µg of whole cell lysate) A: Rat primary hepatocyte 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | Rat primary hepatocyte |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
