Application
PPAR delta antibody [N2C3] detects PPAR delta protein by western blot analysis. A. 30 µg PC-3 whole cell lysate/extract 5% SDS-PAGE PPAR delta antibody [N2C3] dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (50 µg of whole cell lysate) A: Mouse brown adipose tissue 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: Molt-4 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using PPAR delta(beta) antibody at 1:100 dilution.| Product Name | PPAR delta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PPAR delta (peroxisome proliferator-activated receptor delta)This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) family. PPARs are nuclear hormone receptors that bind peroxisome proliferators and control the size and number of peroxisomes produced by cells. PPARs mediate a variety of biological processes, and may be involved in the development of several chronic diseases, including diabetes, obesity, atherosclerosis, and cancer. This protein is a potent inhibitor of ligand-induced transcription activity of PPAR alpha and PPAR gamma. It may function as an integrator of transcription repression and nuclear receptor signaling. The expression of this gene is found to be elevated in colorectal cancer cells. The elevated expression can be repressed by adenomatosis polyposis coli (APC), a tumor suppressor protein related to APC/beta-catenin signaling pathway. Knockout studies in mice suggested the role of this protein in myelination of the corpus callosum, lipid metabolism, and epidermal cell proliferation. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PPAR delta. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Japanese medaka, Dog, Pig, Rabbit, Chicken, BovineRat (94%), Japanese medaka (80%), Dog (98%), Pig (95%), Rabbit (95%), Chicken (90%), Bovine (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FAAR antibody MGC3931 antibody NR1C2 antibody NUC1 antibody NUCI antibody NUCII antibody PPARB antibody PPARD antibody nuclear receptor subfamily 1 group C member 2 antibody nuclear hormone receptor 1 antibody PPAR-beta antibody peroxisome proli |
|---|---|
| Molecular Weight(MW) | 50 kDa (note) (6) |
Application
PPAR delta antibody [N2C3] detects PPAR delta protein by western blot analysis. A. 30 µg PC-3 whole cell lysate/extract 5% SDS-PAGE PPAR delta antibody [N2C3] dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (50 µg of whole cell lysate) A: Mouse brown adipose tissue 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: Molt-4 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using PPAR delta(beta) antibody at 1:100 dilution.| Positive Control | Molt-4 , mouse adipose , PC-3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
