WB
(A) SIRT2 knockdown blocked PPARγ and LXRα, promoted PPARα. The GCs were transfected with SIRT2-siRNA 4 plasmids for 48 h and treated with the SIRT2 inhibitors Thiomyristoyl (2 μM) and SirReal2 (5 μM) for 24 h. Western blotting for PPARα, PPARγ, and LXRα are shown. According to the western blot results, the protein ratios were analyzed in treatment groups relative to the GAPDH protein abundance.(B) SIRT2 knockdown increased Ac-PPARα. Coimmunoprecipitation (coIP) for Ac-PPARα and Ac-PPARγ are shown by using Ac-Lys antibodies. The immunoprecipitates were analyzed by western blotting with the indicated antibodies. IP, immunoprecipitation.| Product Name | PPAR gamma antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PPAR gammaPeroxisome proliferators are non genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPARs). Nuclear hormone receptors are ligand dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase & cytochrome P450 (CYP450) A6 through interaction with specific response elements. The PPAR gamma 2 isoform appears to be induced very early in the differentiation of several cultured adipocyte cell lines, and has been suggested to be a dominant regulator of the murine P2 (aP2) gene which encodes an intracellular lipid binding protein which is expressed only in adipose cells. PPAR gamma 2, like several other nuclear hormone receptors, heterodimerizes with RXR alpha. |
| Immunogen | The peptide used as the immunogen is contained within the region 410 aa to 460 aa from the human PPARgamma protein. |
| Conjugation | Agarose |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB:1:100~1:400 IHC:1:10~1:50 |
|
| Species Reactivity | BovineDogHamsterHumanMousePigRat |
| Concentration | 0.2mg/ml |
| Purification | Protein A |
| Gene Synonyms | NR1C3 PPARG1 PPAR gamma PPARG3 PPARG2 PAX8/PPARG Fusion Gene PPARG Peroxisome Proliferator Activated Receptor gamma HUMPPARG CIMT1 GLM1 PPARgamma |
|---|---|
| Target | PPAR gamma |
| Molecular Weight(MW) | 58 kDa |
| Tissue Specificity | will recognize both PPARgamma1 and PPARgamma2 |
| Cellular Localization | Nuclear |
WB
(A) SIRT2 knockdown blocked PPARγ and LXRα, promoted PPARα. The GCs were transfected with SIRT2-siRNA 4 plasmids for 48 h and treated with the SIRT2 inhibitors Thiomyristoyl (2 μM) and SirReal2 (5 μM) for 24 h. Western blotting for PPARα, PPARγ, and LXRα are shown. According to the western blot results, the protein ratios were analyzed in treatment groups relative to the GAPDH protein abundance.(B) SIRT2 knockdown increased Ac-PPARα. Coimmunoprecipitation (coIP) for Ac-PPARα and Ac-PPARγ are shown by using Ac-Lys antibodies. The immunoprecipitates were analyzed by western blotting with the indicated antibodies. IP, immunoprecipitation.| Positive Control | 3T3-L1 |
|---|---|
| Application Notes | WB:1:100~1:400 IHC:1:10~1:50 |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Preservative: 0.1% Sodium Azide. Constituents: 0.2% Gelatin, PBS |
